Composite

Part:BBa_K2057003:Design

Designed by: José Eleazar Barboza Corona   Group: iGEM16_Guanajuato_Mx   (2016-10-17)


LasR under the regulation of the promoter chiA74


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 489
    Illegal XbaI site found at 506
    Illegal NgoMIV site found at 896
    Illegal AgeI site found at 1093
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We previously tested the functionality of the promoter chiA74 in E. coli


Source

Both regulatory elements comes from regulatory elements

References

(i) BARBOZA-CORONA JE, Delgadillo-Ángeles JL, Castañeda-RamĂ­rez JC, Barboza-PĂ©rez UE, Casados-VĂĄzquez LE, Bideshi DK, del RincĂłn-Castro MC (2014) Bacillus thuringiensis subsp. kurstaki HD1 as a factory to synthesize alkali-labile ChiA74∆sp chitinase inclusions, Cry crystals and spores for applied use. Microbial Cell Factories. 13:15. doi:10.1186/1475-2859-13-15 (ii) BARBOZA-CORONA JE, Nieto-Mazzocco E, VelĂĄzquez-Robledo R, Salcedo-HernĂĄndez R, Bautista M, JimĂ©nez B, Ibarra JE (2003) Cloning, sequencing and expression of the chitinase gene chiA74 from Bacillus thuringiensis. Applied and Environmental Microbiology. 69(2): 1023-1029.